Northern Territory Police have arrested a man following a serious aggravated assault at a Yulara hotel early this morning.

Around 4:38am, police received a report from onsite security staff at the hotel that a woman had suffered a significant hand injury after being attacked with a knife.

Police attended and established that a 53-year-old man had allegedly entered a hotel room occupied by a 57-year-old woman and a 24-year-old woman, all of whom are known to each other.

The victims asked the man to leave, at which point he is alleged to have become aggressive and armed himself with a knife.

He then allegedly stabbed the 24-year-old woman in the right forearm, causing a deep laceration.

The older victim attempted to intervene and grabbed the blade, receiving serious injuries to her left hand and fingers.

The offender was arrested at the scene.

Both victims were taken to the local health clinic, with the 57-year-old woman flown to Alice Springs for surgery.

A crime scene has been declared, and investigations are ongoing.

Police urge anyone who witnessed the disturbance or has information that may assist with the investigation to contact Police on 131 444 and quote reference number NTP2500120683 or report anonymously through Crime Stoppers on 1800 333 000 or via crimestoppers.com.au.